聚乙二醇200 基硝苯柳胺与钥孔戚血蓝蛋白的相互作用
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[4 ]Guo W ,Zheng L Y,Wu Y Q ,Fan X L. Chin. Chem. Lett . [J ] ,2008 ,19 :406.
[5 ] Sulkowsak A ,Równicka J ,Bojko B. J . Mol. St ruct . [J ] ,2003 ,651～653 :133.
[6 ] L IU J ia2qin (刘家琴) , TIAN J ian2niao (田建袅) ,BIAN Qing2quan (边清泉) , HU Zhi2de (胡之德) . Spectroscopy andSpectral Analysis (光谱学与光谱分析) [J ] ,2006 ,26 (4) :716.
[7 ] CHEN Guo2zhen (陈国珍) ,HUAN G Xian2zhi (黄贤智) ,ZHEN G Zhu2zi (郑朱梓) . Methods of Fluorescence Analysis(荧光分析法) [M] . Beijing (北京) :Science Press (科学出版社) ,1990 ,112.
[8 ] XIE M X ,LON G M ,L IU Y. Biochim. Biophys.Acta[J ] ,2006 ,1760 :1184.
[9 ] MA Jing (马静) ,ZHENG Xue2fang (郑学仿) , TAN G Qian (唐乾) , YAN G Yan2jie (杨彦杰) ,SUN Xia (孙霞) ,GAO Da2bin (高大彬) . Chem. J . Chinese Universities (高等学校化学学报) [J ] ,2008 ,29 (2) :260.
[10 ] BAI Hai2xin (白海鑫) ,YAN G Cheng (杨 成) , YAN G Xiu2rong (杨秀荣) . Chem. J . Chinese Universities (高等学校化学学报) [J ] ,2007 ,28 (2) :227.
[11 ] MA Xu2wen (马旭文) ,L I Zhong2ping (李忠平) ,L I J ian2qing (李建晴) ,DONG Chuan (董川) . Journal of AnalyticalScience (分析科学学报) [J ] ,2008 ,24 (4) :399.
[12 ] Il’ichev Y V ,Perry J L ,Simon J D. J . Phys. Chem. B[J ] ,2002 ,106 :452.
[13 ] L IU Gen2lan (刘根兰) ,NI Yong2nian (倪永年) . Chem. J . Chinese Universities (高等学校化学学报) [J ] ,2008 ,29 (7) :1342.
[14 ] Haron M K,Thomas J J ,Nicholas C P. Biochimica et BiophysicaActa[J ] ,2005 ,1751 :119.
[15 ]Dockal M ,Carter D C ,Ruker F. J . Biol. Chem. [J ] ,2000 ,275 (5) :3042.
Interaction of Keyhole Limpet Hemocyanin with
PEG2002niphensamide
WU Yong2quan , GUO Wei , XU Li2rong , TAN G Biao2sheng ,
ZHAO J iao2jiao , ZHEN G Lu2
yin , FAN Xiao2lin 3
( Key L aboratory of Organo2p harmaceutical Chemist ry of J i an g x i Province , Gannan N ormal
Universi t y ,Ganzhou341000)
Abstract :The interaction of keyhole limpet hemocyanin ( KL H ) and PEG2002nip hensamide was
investigated by fluorescence spectroscopy , ultraviolet absorption spectroscopy and circular dichroism(CD) spectroscopy. It was shown that KL H fluorescence at 340 nm was quenched regularly wit h theaddition of PEG2002nip hensamide ; t he quenching constant s were decreased with the temperatureincreasing ; and the quenching was verified as static fluorescence quenching. The binding constant s ( K)were obtained according to Lineweaver2Burk equation at different temperature. The calculatedthermodynamic parameter s ( △H , △S) according to Van’t Hoff equation indicated t hat the elect rostaticinteraction played major roles in t he binding processes. The distance between KL H and PEG2002nip hensamide was 5. 76 nm according to t he theory of the FÊrster energy t ransference. The effect s ofPEG2002nip hensamide on the conformation of KL H were f urt her analyzed by synchronous fluorescencespectroscopy and circular dichroismspectroscopy. PEG2002nip hensamide could be deposited andtransported by KL H and it affected t he conformation of KL H. The secondary structure of KL H wasaltered (CD data) , i. e. , t heα2helices were reduced f rom 43. 1 % to 37. 8 %.
Keywords :Nip hensamide ; Keyhole limpet hemocyanin ; Fluorescence spectroscopy ; Ultraviolet
spectroscopy ; Circular dichroismspectrometry
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